@InCollection{LoboAnPaPaCoTrCo:2009:InViAs,
               author = "Lobo, Anderson de Oliveira and Antunes, Erica Freire and Palma, 
                         Mariana Bernardes da Silva and Pacheco-Soares, Cristina and Corat, 
                         Marcus Alexandre Finzi and Trava-Airoldi, Vladimir Jesus and 
                         Corat, Evaldo Jose",
                title = "Biocompatibility differences between dispersed and 
                         vertically-aligned carbon nanotubes: An in vitro assays review",
            booktitle = "Carbon nanotubes: new research",
            publisher = "Nova Science Publishers",
                 year = "2009",
               editor = "Ottenhouse, A. P.",
                pages = "281--316",
             keywords = "Nanotubos de Carbono, Compatilidade Biol{\'o}gica.",
             abstract = "An overview about carbon nanotube (CNT) production and quality 
                         parameters will be presented, as well a review of current 
                         literature about in vitro assays commonly used to evaluate the 
                         biocompatibility of CNT. The limits of colorimetric assays for 
                         CNTs evaluation will be discussed, using comparisons between 
                         dispersed CNT and CNT arrays. The influence of nanotopography and 
                         wettability of CNT scaffolds for cell adhesion will be shown. 
                         Studies carried out in our laboratories with vertically-aligned 
                         carbon nanotubes (VACNT) will also be presented. We have shown the 
                         interaction among CNT (VACNT) and four cell lines: mouse 
                         fibroblasts (L-929), mouse embryo fibroblast (C57/BL6) with or 
                         without green fluorescent protein (GFP) and human osteoblast 
                         (SaOS-2). The biocompatibility tests were performed with in vitro 
                         tests on raw-VACNT and after superficial modification by O2 
                         plasma, which changes its hydrophobic character. The non-toxicity, 
                         cell viability, proliferation and cell adhesion were evaluated by: 
                         (i) 2-(4,5-dimethyl-2-thioazoly)-3,5-diphenyl-2H-tetrazolium 
                         bromide (MTT) assay; (ii) Lactate dehydrogenase (LDH) assay; (iii) 
                         neutral red (NR) assay; (iv) Scanning electron microscopy (SEM); 
                         and fluorescence microscopy. The influence of catalyst type,VACNT 
                         density and superficial modification were evaluated by 
                         morphological, structural and superficial techniques: SEM, 
                         Transmission electron microscopy (TEM), Raman spectroscopy, 
                         contact angle (CA) and X-Ray Photoelectron Spectroscopy (XPS). 
                         High cell viability, exceptional cell adhesion and preference were 
                         achieved.",
          affiliation = "{Universidade do Vale do Para{\'{\i}}ba (UNIVAP)} and {Instituto 
                         Nacional de Pesquisas Espaciais (INPE)} and {} and {} and {} and 
                         {Instituto Nacional de Pesquisas Espaciais (INPE)} and {Instituto 
                         Nacional de Pesquisas Espaciais (INPE)}",
                 isbn = "978-1-60692-2",
                label = "lattes: 0560039556461253 7 LoboAnPaPaCoTrCo:2009:InViAs",
             language = "en",
           targetfile = "lobo_biocompatibility.pdf",
               volume = "278",
        urlaccessdate = "13 maio 2024"
}